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B.Sc. Biology

B.Sc. Biology – Department of Biochemistry (Prof. Dr. Bettina Warscheid)

For more details and enrolling visit Campus Management HISinOne
link (you can search for the number of the module or course).
 

 

 Basic Module (GM-14): „Microbiology, Immunology, Biochemistry“, Lecture: „Basics of Biochemistry“

4th semester; lecture: 2.5 SWS

Content: The lecture provides basic knowledge in biochemistry - amino acids, structure and function of proteins, biochemical methods for protein analysis, function and regulation of proteins/enzymes, enzyme kinetics, signal transduction mechanisms, glycolysis and gluconeogenesis, citric acid cycle, oxidative phosphorylation, glycogen and fatty acid metabolism.

 
 

 Profile Module (PM-12): „Biochemistry – Introduction to Functional Proteomics“

4th semester; lecture, exercise, and seminar:  4 SWS

(currently not offered this semester)

Content: Based on proliferating and differentiating muscle cells as biological system under study, this module provides the students with basic knowledge and hands-on experience in biochemical methods and differential proteomics techniques employing 2-D gel electrophoresis. The lecture covers topics ranging from the (patho-)biochemistry of muscles, signaling pathways regulating proliferation and differentiation of muscle cells, posttranslational modifications, protein-interaction studies, function and reactions of proteases to fundamental protein analytical methodologies, 2-D gel electrophoresis, techniques based on mass spectrometry (MALDI/ESI) and bioinformatics approaches for data analysis.

 
 

 Advanced Module (VM-01): „Biochemistry – Synthetic Biology and Proteomics“

5th semester; lecture, exercise, and seminar:  7 SWS

Content: The lecture conveys in-depth knowledge on protein analytical techniques including protein mass spectrometry. The practical part provides hands-on experience in protein fractionation techniques, enzyme kinetics, isoelectric focusing, size exclusion chromatography and HPLC, ion exchange chromatography, affinity chromatography, fluorescence resonance energy transfer (FRET), mammalian cell culture techniques including transfection, analysis of protein expression in mammalian cells using SDS-PAGE and Western blotting.

  

 Project Module: „Biochemistry – Functional Proteomics“

5th semester; exercise and seminar: 4 SWS (two-week course)

Content: Theoretical and practical introduction to fundamental and advanced protein analytical, cell biological and molecular biological techniques as well as (quantitative) proteomics strategies as preparation for the bachelor thesis. The methods taught depend on the topic of the individual bachelor thesis and comprise a large array of biochemical, cell and molecular biological as well as quantitative/functional proteomics techniques, high resolution mass spectrometry and bioinformatics/statistics such as cultivation of bacteria, yeast and mammalian cells; recombinant expression and subsequent purification of proteins using the ÄKTA protein purification system; transfection or transduction of mammalian cells; SDS-PAGE and (quantitative) Western blotting; subcellular fractionation and purification of organelles; protein and peptide fractionation by strong cation/anion exchange, reversed-phase and size exclusion chromatography; kinase and phosphatase assays; affinity purification, co-immunoprecipitation and BioID for the analysis of protein-protein interactions; fluorescence microscopy; strong cation exchange (SCX) and TiO2 chromatography for the enrichment of phosphopeptides; in vitro and in vivo chemical and UV-crosslinking of proteins for structural analyses; LC/MS/MS analyses using different fragmentation techniques (CID, MSA, ETD, HCD); and bioinformatics and statistical analyses of MS/MS data.

 

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