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M.Sc. Biology

M.Sc. Biology – Department of Biochemistry (Prof. Dr. Bettina Warscheid)

For more details and enrolling visit Campus Management HISinOne link (you can search for the number of the module or course).

 

 Orientation Module (OM-01): "Translational Biology"

(Major: Translational Biology)

1st semester; lecture and exercise within this orientation module: 1.5 SWS

Content: The "Functional Proteomics" part of this module provides in-depth knowledge about the biochemistry of glycoproteins ranging from biosynthesis and general functions in a cellular context to their analysis and significance as biopharmaceuticals. The lecture covers mechanisms of intracellular protein sorting, structure and synthesis of oligosaccharide chains, N- and O-glycosylation, significance of protein glycosylation in general and as therapeutics as well as strategies for glycoanalytics and glyoproteomics. The practical part provides hands-on experience in the MS-based analysis of glycoproteins including gel-based LC/MS sample preparation and analysis of high resolution MS data.      

 

 

 Orientation Module (OM-04): "Biochemistry and Microbiology"

(Major: Biochemistry and Microbiology)

1st semester; lecture and exercise within this orientation module: 3 SWS

Content: The part "Biochemistry" of this module focuses on the regulation of the carbon metabolism of the eukaryotic model organism Saccharomyces cerevisiae. The lecture provides in-depth knowledge about the carbon and amino acid metabolism in S. cerevisiae including glycolysis, citric acid cycle, respiration, fermentation, the regulation of biochemical pathways, metabolite repression/induction and glucose signaling networks. Further topics covered are genetics and molecular biology of S. cerevisiae as well as basic and new approaches for protein analysis in yeast. The practical part includes cultivation and growth tests of wild-type and mutant yeast strains, the analysis of differential protein expression in wild-type and mutant yeast using both "classical" biochemical techniques (SDS-PAGE and immunoblotting) and gel-enhanced high resolution MS as well as the determination of substrate-specific enzyme activities.      

 

 

 

 Major Module 1 (SP1-01): "Quantitative Methods"

(Major: Translational Biology)

2nd semester; exercise and seminar within this major module: 1 SWS

Content: This module aims at learning and applying the programming language „Python“ for the quantitative description of biological systems, among others covering applications in the field of quantitative proteomics, e.g. the visualization of SILAC-based protein interaction data. The module further provides knowledge about the basic principles of protein identification by mass spectrometry, discovery proteomics workflows, quantitative mass spectrometry and strategies for protein quantification, the analysis of posttranslational protein modifications as well as the analysis of protein-protein interactions.      

 

 

 

 Major Module (SP1-04): "Microbiology and Systems Biochemistry"

(Major: Biochemistry und Microbiology)

2nd semester; exercise and seminar within this major module: 1.5 SWS

Content: The "Biochemistry" part of this module covers organellar biochemistry and function from yeast to human with focus on mitochondria and peroxisomes. Topics of the lecture include principles and mechanisms of organellar biogenesis and degradation, metabolic functions, protein import, diseases associated with organellar dysfunction as well as strategies in quantitative and functional yeast proteomics. The exercise provides hands-on experience in metabolic labeling of yeast cells using SILAC, the isolation of yeast organelles by differential centrifugation, the analysis of auxotrophic and knock-out yeast strains, global quantitative proteomics using high resolution MS as well as bioinformatics data analysis and visualization.      

 

 

 Elective Module (WM-17): "Signaling in Tumor Cells - Functional Proteomic Studies"

3rd semester; lecture, exercise and seminar: 7 SWS

Content: The lecture provides a comprehensive overview about signaling pathways in health and disease, the function and regulation of protein kinases and phosphatases, protein kinases as targets in tumor therapy, tumor stem cells, novel approaches for analyzing posttranslational protein modifications as well as quantitative and phosphoproteomics strategies combined with bioinformatics for the study of cell signaling. The practical part includes handling, cultivation and transfection of mammalian cells, fluorescence microscopy, expression and analysis of protein kinases by Western blot analysis, inhibition of signaling pathways in breast cancer cells, purification and MS-based detection of phosphoproteins, deciphering protein networks by affinity purification-MS and bioinformatics data analysis.      

 

 

 

 Major Module 2 (SP2-06): "Functional Proteomics and Biochemistry"

3rd semester; lecture, exercise and seminar: 15 SWS

Content: The lecture provides insights into biochemical and cell biological as well as advanced functional proteomic techniques and strategies applied to biological and disease-related questions. The practical part consists of lab work addressing a defined project in the field of cell biology using a large array of biochemical, cell and molecular biological as well as quantitative/functional proteomics techniques, high resolution mass spectrometry and bioinformatics/statistics for data analysis. The seminar includes comprehensive workshops on “Scientific Writing” and “Project Management“.
The students have to write a short project proposal in preparation of their Master thesis project. Proposals are evaluated and discussed with the students.

 

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